The time order of autophagy and apoptosis process in the oxidative injury in glioma U251 cells.
- Author:
Xiao-Xia KONG
1
;
Hong-Yu ZHANG
;
Ran CHEN
;
Xiao-Fang FAN
;
Yong-Sheng GONG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; physiology; Autophagy; physiology; Brain Neoplasms; pathology; Cell Line, Tumor; Glioma; pathology; Humans; Oxidative Stress; physiology; Time Factors
- From: Chinese Journal of Applied Physiology 2011;27(4):471-474
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the time order of autophagy and apoptosis in human U251 cells injury after H2O2 treatment.
METHODS4 groups in this study were set up, normal control group, 1 mmol/L H2O2 (6 h,12 h, 24 h) group. The viability of U251 cells treated with H2O2 was measured by MTT assay. Cell apoptotic ratio was determined by flow cytometry analysis. Autophagic vacuoles were stained with monodansylcadaverine. The protein level of Beclin 1 and cytosolic cyt c were assayed by using Western blot.
RESULTSCompared with the control group, cell viability decreased significantly under 1 mmol/L H2O2 treatment in time-dependent way. Autophagic vacuoles and the expression of autophagic protein Beclin 1 increased at 6 h, but cell apoptotic ratio and cytosolic cyt c protein did not change obviously, cell apoptotic ratio and cytosolic cyt c protein level increased at 12 h and 24 h (P < 0.05).
CONCLUSIONOxidative stress induced autophagy and apoptosis in U251 glioma cells, and autophagy eventuated ahead of apoptosis.
