Preliminary study about the role of c-src in the initiation of primordial follicle in rat ovary.
- Author:
Xiao-Yu DU
1
;
Dan-Feng TANG
;
Jian HUANG
;
Yuan-Hui XU
;
Wei-Yun CHEN
;
Li-Ping ZHENG
;
Yue-Hui ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Base Sequence; Culture Techniques; Female; Molecular Sequence Data; Ovarian Follicle; growth & development; metabolism; Ovary; growth & development; metabolism; Proto-Oncogene Proteins pp60(c-src); genetics; physiology; RNA Interference; RNA, Messenger; genetics; metabolism; RNA, Small Interfering; genetics; Rats; Rats, Sprague-Dawley; Transfection
- From: Chinese Journal of Applied Physiology 2011;27(4):490-494
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of c-src on the initiation of primordial follicles.
METHODS2-days-old female SD rats' ovaries were cultured in Waymouth culture system and were used HE staining and immunohistochemy to observe the number of follicles after 0, 4, 8 days cultured. Use chemically synthesized small interference RNA (siRNA) transfected into ovarian tissue in cultured for RNA interference, and use HE staining and RT-PCR to detect the best siRNA and packaging it by lentiviruses to test the interference effect.
RESULTSWith the increase of culturing days, the nummber of the primordial follicles in ovarian gradually reduced. We packed the best siRNA by lentiviruses to doing RNA interference and found comparing with the blank control group and blank vector group, c-src mRNA of the best interference group were significantly decreased. The total number of primordial follicles was relatively greater and the development of primordial folliculars was inhibited.
CONCLUSIONc-src plays an important role in primordial follicle development and folliculogenesis initiation.
