Differential expression of alpha-adrenoceptor subtypes in rat dorsal root ganglion after chronic constriction injury.
10.1007/s11596-014-1277-1
- Author:
Hong-ju CHENG
1
;
Ke-tao MA
;
Li LI
;
Lei ZHAO
;
Yang WANG
;
Jun-qiang SI
Author Information
1. Department of Physiology, Basic Medical School of Wuhan University, Wuhan, 430071, China, chenghongju1975@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Size;
Chronic Disease;
Constriction, Pathologic;
Fluorescent Antibody Technique;
Ganglia, Spinal;
metabolism;
pathology;
Male;
Microscopy, Confocal;
Neurons;
metabolism;
pathology;
Pain Measurement;
methods;
Pain Threshold;
Protein Isoforms;
metabolism;
Rats;
Rats, Sprague-Dawley;
Receptors, Adrenergic, alpha-1;
metabolism;
Receptors, Adrenergic, alpha-2;
metabolism;
Sciatic Nerve;
injuries;
surgery
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(3):322-329
- CountryChina
- Language:English
-
Abstract:
mRNAs of alpha-adrenoceptor (α-AR) subtypes are found in neurons in dorsal root ganglion (DRG) and change after peripheral nerve injury. In this study, the distribution of α-AR subtype proteins was studied in L5 DRG of normal rats and rats with chronic constriction injury of sciatic nerve (CCI). Using immunofluorescence technique, it was found that α1A-, α1B-, and α2A-AR proteins were expressed in large, medium, and small size neurons in normal DRG, and significantly increased in all size neurons 14 days after CCI. α1D- and α2C-AR was also expressed in all size neurons in normal DRG. However, α1D-AR was significantly increased and α2C-AR was decreased in small size neurons 14 days post CCI. α2B-AR neurons were not detectable in normal and CCI DRG. Co-expression of α1A- and α2A-AR in the same neuron was observed in normal DRG and increased post CCI. Collectively, these results indicated that there is distinct distribution of α-AR subtypes in DRG neurons, and the distribution and levels of expression of α-AR subtypes change differently after CCI. The up-regulation of α-AR subtypes in DRG neurons may play an important role in the process of generating and transmitting neuropathic pain.
