Changes of c-fos, malondialdehyde and lactate in brain tissue after global cerebral ischemia under different brain temperatures.
10.1007/s11596-014-1282-4
- Author:
Hong ZHANG
1
;
Li LI
;
Guo-ying XU
;
Yuan-wu MEI
;
Jun-jian ZHANG
;
Shen-xing MURONG
;
Sheng-gang SUN
;
E-tang TONG
Author Information
1. Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China, zhangh9@yahoo.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Body Temperature;
Brain;
blood supply;
metabolism;
physiopathology;
Brain Ischemia;
metabolism;
physiopathology;
Cerebral Cortex;
blood supply;
metabolism;
physiopathology;
Hippocampus;
blood supply;
metabolism;
physiopathology;
Immunochemistry;
Lactic Acid;
metabolism;
Male;
Malondialdehyde;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Proto-Oncogene Proteins c-fos;
metabolism;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
metabolism;
physiopathology;
Spectrophotometry;
Temperature;
Time Factors;
Tumor Suppressor Protein p53;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(3):354-358
- CountryChina
- Language:English
-
Abstract:
Under global cerebral ischemia, the effect of different brain temperature on cerebral ischemic injury was studied. Male Sprague-Dawley rats were divided into normothermic (37-38°C) ischemia, mild hypothermic (31-32°C) ischemia, hyperthermic (41-42°C) ischemia and sham-operated groups. Global cerebral ischemia was established using the Pulsinelli four-vessel occlusion model and brain temperature was maintained at defined level for 60 min after 20-min ischemia. The expression of c-fos protein and the levels of malondialdehyde (MDA) and lactate in brain regions were detected by immunochemistry and spectrophotometrical methods, respectively. C-fos positive neurons were found in the hippocampus and cerebral cortex after cerebral ischemia reperfusion. Mild hypothermia increased the expression of c-fos protein in both areas, whereas hyperthermia decreased the expression of c-fos protein in the hippocampus at 24 h reperfusion, and the cerebral cortex at 48 h reperfusion when compared to normothermic conditions. In normothermic, mild hypothermic and hyperthermic ischemia groups, the levels of MDA and lactate in brain tissue were increased at 24, 48 and 72 h reperfusion following 20-min ischemia as compared with the sham-operated group (P<0.01). The levels of MDA and lactate in mild hypothermic group were significantly lower than those in normothermic group (P<0.01). It is suggested that brain temperature influences the translation of the immunoreactive protein product of c-fos after global cerebral ischemia, and MDA and lactate are also affected by hypothermia and hyperthermia.
