Downregulation of LncRNAH19 and MiR-675 promotes migration and invasion of human hepatocellular carcinoma cells through AKT/GSK-3β/Cdc25A signaling pathway.
10.1007/s11596-014-1284-2
- Author:
Jun LV
1
;
Ling MA
;
Xi-lin CHEN
;
Xiao-hui HUANG
;
Qian WANG
Author Information
1. Department of Hepatobiliary Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China, lvjun1967@sina.com.
- Publication Type:Journal Article
- MeSH:
Blotting, Western;
Carcinoma, Hepatocellular;
genetics;
metabolism;
pathology;
Cell Line;
Cell Line, Tumor;
Cell Movement;
genetics;
Down-Regulation;
Gene Expression Regulation, Neoplastic;
Gene Knockdown Techniques;
Glycogen Synthase Kinase 3;
metabolism;
Glycogen Synthase Kinase 3 beta;
Hep G2 Cells;
Humans;
Liver Neoplasms;
genetics;
metabolism;
pathology;
MicroRNAs;
genetics;
Neoplasm Invasiveness;
Proto-Oncogene Proteins c-akt;
metabolism;
RNA Interference;
RNA, Long Noncoding;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Signal Transduction;
cdc25 Phosphatases;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(3):363-369
- CountryChina
- Language:English
-
Abstract:
LncRNAH19 has been implicated as having both oncogenic and tumor suppression properties in cancer. LncRNAH19 transcripts also serve as a precursor for miR-675. However, it is unknown whether LncRNAH19 and miR-675 are involved in the migration and invasion of hepatocellular carcinoma (HCC) cells. The purpose of this study was to investigate the effect and mechanism of LncRNAH19 and miR-675 on migration and invasion of HCC cells. The migration and invasion of HCC cells were measured by Transwell migration and invasion assays after transfection of HCC cells with miR-675 inhibitors and LncRNAH19siRNA. The levels of LncRNAH19 and miR-675 were detected by quantitative reverse transcriptase real-time polymerase chain reaction (qRT-PCR), and the protein expression of AKT, GSK-3β and Cdc25A by Western blotting analysis. The expression levels of LncRNAH19 and miR-675 were higher in MHCC-97H cells than in L02, Huh-7 and HepG2 cells. Transwell migration assay revealed that the miR-675 inhibitor and LncRNAH19siRNA could significantly increase the migration of HCC cells (P<0.01) as compared with the control group. Transwell invasion assay demonstrated that the miR-675 inhibitor and LncRNAH19siRNA could significantly increase the invasion of HCC cells (P<0.01) as compared with the control group. Western blotting analysis showed that the expression levels of AKT and Cdc25A were significantly increased (P<0.05), and the expression level of GSK-3β was significantly decreased (P<0.05) after treatment with miR-675 inhibitors and LncRNAH19siRNA as compared with the control group. These findings suggested that inhibition of LncRNAH19 and miR-675 expression can promote migration and invasion of HCC cells via AKT/GSK-3β/Cdc25A signaling pathway.
