Effect of complement C1q expression on hepatic ischemia-reperfusion injury in rats.
10.1007/s11596-014-1291-3
- Author:
Xiao-bo FENG
1
;
Jian-juan KE
;
Yan RAO
;
Zong-ze ZHANG
;
Yan-lin WANG
Author Information
1. Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China, 1219628972@qq.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Blotting, Western;
Complement C1q;
genetics;
metabolism;
Gene Expression;
Liver;
blood supply;
metabolism;
Male;
Malondialdehyde;
metabolism;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
physiopathology;
Reverse Transcriptase Polymerase Chain Reaction;
Superoxide Dismutase;
metabolism;
Time Factors
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(3):403-407
- CountryChina
- Language:English
-
Abstract:
The effect of the complement C1q expression on total hepatic ischemia-reperfusion (I/R) injury in rats was investigated. Sixty healthy male Sprague Dawley (SD) rats weighing 180-200 g were randomly divided into 5 groups: sham-operation group (S group, n=12); group of I/R for 1 h (I/R 1 h group, n=12); group of I/R for 3 h (I/R 3 h group, n=12); group of I/R for 6 h (I/R 6 h group, n=12); group of I/R for 24 h (I/R 24 h group, n=12). The hepatic I/R model of rats was established, and liver tissues were obtained 1 h, 3 h, 6 h and 24 h after hepatic I/R, respectively. Furthermore, the tissues were stained using hematoxylin-eosin, and the liver injuries of rats were observed using a microscope. The malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in liver tissue were determined. Real-time polymerase chain reaction (PCR) and Western blotting were used to detect the expression levels of C1q mRNA and protein, respectively. As compared with the S group, the histopathological changes in I/R 1 h-24 h groups were gradually aggravated with the extension of I/R time. As compared with the S group, SOD activity and MDA content in the I/R groups were reduced and increased respectively with the extension of I/R time (P<0.01). Furthermore, the C1q expression at mRNA and protein levels in the I/R groups (especially in the I/R 3 h group) was significantly higher than that in the S group (P<0.05). It is suggested that C1q expression may play a principal role in hepatic I/R injury, particularly at the early stage of perfusion.
