Expression profiling of microRNAs in hippocampus of rats following traumatic brain injury.
10.1007/s11596-014-1313-1
- Author:
Ting-yi SUN
1
;
Xiao-rui CHEN
;
Zi-long LIU
;
Li-li ZHAO
;
Yong-xiang JIANG
;
Guo-qiang QU
;
Rong-shuai WANG
;
Si-zhe HUANG
;
Liang LIU
Author Information
1. Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, sty-j211@sohu.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Biomarkers;
metabolism;
Brain Injuries;
metabolism;
pathology;
Female;
Forensic Genetics;
Gene Expression Profiling;
Gene Expression Regulation;
Hippocampus;
metabolism;
pathology;
Male;
MicroRNAs;
biosynthesis;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(4):548-553
- CountryChina
- Language:English
-
Abstract:
The changes of microRNA expression in rat hippocampus after traumatic brain injury (TBI) were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI: 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regulated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.
