Analysis of Th1/Th2 response pattern for erythrodermic psoriasis.
10.1007/s11596-014-1322-0
- Author:
Ping ZHANG
1
;
Hong-xiang CHEN
;
Yi-qun DUAN
;
Wei-zhen WANG
;
Tian-zhu ZHANG
;
Jia-wen LI
;
Ya-ting TU
Author Information
1. Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, hautarzt2010@aliyun.com.
- Publication Type:Clinical Trial
- MeSH:
Adult;
Cytokines;
immunology;
Dermatitis, Exfoliative;
immunology;
pathology;
Female;
Gene Expression Regulation;
immunology;
Humans;
Male;
Psoriasis;
immunology;
pathology;
Skin;
immunology;
pathology;
Th1 Cells;
immunology;
pathology;
Th2 Cells;
immunology;
pathology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(4):596-601
- CountryChina
- Language:English
-
Abstract:
As one of the most serious types of psoriasis, pathogenesis of erythrodermic psoriasis (EP) is unclear so far. In this study, we aimed to detect the levels of Th1/Th2 cytokine-associated transcription factors and T-lymphocyte clone in peripheral blood mononuclear cells (PBMCs) derived from EP patients, and gene expression level of T-bet/GATA-3 in skin lesion. The potential role of Th1/Th2 reaction pattern played in the pathogenesis of EP was also discussed. Serum levels of IFN-γ, IL-2, IL-4 and IL-10 were quantified by ELISA among 16 EP patients, 20 psoriasis vulgaris (PV) patients and 15 healthy controls. The expression levels of T-bet/GATA-3 in the skin lesion and PBMCs were examined by real-time qPCR. The ratio of Th1/Th2 was measured by flow cytometry. The levels of IFN-γ, IL-2, IL-4 and IL-10 were higher in EP patients than in the healthy controls. The levels of IL-4 and IL-10 were 69.44±11.45 and 12.62±4.57 pg/mL, respectively, in EP patients, significantly higher than those in PV patients and healthy controls (P<0.05). Flow cytometry revealed the levels of both Th1 and Th2 in PBMCs from EP patients were higher than those in healthy controls, and the Th1/Th2 ratio was dramatically lower than in PV patients (P<0.01). The ratios of IFN-γ/IL-4 and T-bet/GATA-3 in EP patients were both less than 1.0, suggesting a reversal when compared with the other two groups. Our study indicated that the EP patients exerted a Th1/Th2 bidirectional response pattern, and the balance of Th cell subsets inclines to Th2, which might be one of the important mechanisms of EP pathogenesis.
