Activation of PPAR-γ inhibits differentiation of rat osteoblasts by reducing expression of connective tissue growth factor.
10.1007/s11596-014-1332-y
- Author:
Wei-Wei YU
1
;
Qin XIA
;
Yan WU
;
Qiao-Yun BU
Author Information
1. Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, 77720522@qq.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Blotting, Western;
Cell Differentiation;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Connective Tissue Growth Factor;
genetics;
metabolism;
Dose-Response Relationship, Drug;
Gene Expression;
drug effects;
Hypoglycemic Agents;
pharmacology;
Osteoblasts;
cytology;
drug effects;
metabolism;
PPAR gamma;
agonists;
metabolism;
Rats, Sprague-Dawley;
Reverse Transcriptase Polymerase Chain Reaction;
Thiazolidinediones;
pharmacology;
Transforming Growth Factor beta1;
pharmacology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(5):652-656
- CountryChina
- Language:English
-
Abstract:
Long-term treatment with an agonist of peroxisome proliferator-activated receptor (PPAR)-γ is associated with bone fractures in the clinical practice. However, the mechanisms underlying the fractures are not fully understood. This study was aimed to examine the effect of rosiglitazone (an agonist of PPAR-γ) of different doses on the proliferation, differentiation, and transforming growth factor beta 1 (TGF-β1)-induced expression of connective tissue growth factor (CTGF) in primary rat osteoblasts in vitro. Osteoblasts were isolated from newly born SD rats and treated with different doses of rosiglitazone (0-20 μmol/L). The proliferation and differentiation of osteoblasts were measured by MTT assay and NPP assay, respectively. The expression of CTGF was determined by RT-PCR and Western blotting. The results showed that most isolated osteoblasts displayed strong alkaline phosphatase (ALP) activity and treatment with different doses of rosiglitazone did not affect their proliferation, but significantly inhibited the differentiation of osteoblasts in a dose-dependent manner. Moreover, treatment with different doses of rosiglitazone significantly reduced the TGF-β1-induced CTGF mRNA transcription and protein expression in a dose-dependent manner in rat osteoblasts. It was concluded that the activation of PPAR-γ may inhibit the differentiation of osteoblasts by reducing the TGF-β1-induced CTGF expression in vitro.
