Determination of triterpene delta-amyrone in Sedum sarmentosum.
- Author:
Su-ping CAO
1
;
Li-fang LIU
;
Qiang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Plants, Medicinal; chemistry; Quality Control; Sedum; chemistry; Triterpenes; analysis
- From: China Journal of Chinese Materia Medica 2006;31(1):41-50
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the quantitative analysis method of triterpene delta-amyrone in Sedum sarmentosum by HPLC-ELSD.
METHODThe Zorbax Sil Column was used and the mobile phase was dichloromethane-methanol (99:1). The flow rate was set at 0.5 mL x min(-1), the temperature of drift tube was set at 100 degrees C and the nebulizer nitrogen flow rate was 3.0 L x min(-1).
RESULTThe linear range of sigma-amyrone was 2.12-10.6 microg. The average recovery was 100.5%.
CONCLUSIONThe results showed that the method was reliable, simple and precise. It would be beneficial for the study of the quality control of S. sarmentosum.
