Determination of epicatechin in Rhizoma Fagopyri dibotoryis by HPLC-ECD.
- Author:
Min-Tong XIN
1
;
Bin YANG
;
Hua LI
;
Lu-Qi HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Catechin; analysis; Chromatography, High Pressure Liquid; methods; Electrochemistry; Fagopyrum; chemistry; Plants, Medicinal; chemistry; Reproducibility of Results; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2006;31(2):117-119
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a quantitative method of determination of epicatechin in Rhizoma fagopyri dibotoryis by HPLC-electrochemical detection.
METHODThe sample was separated on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol-0.1 mol x L(-1) phosphate buffer (1:3, pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the applied potential was + 600 mV.
RESULTThe linear range of epicatechin was 0.004 875-1.56 microg, r = 0.999 9. The average recovery was 100.7% with RSD 1.9% (n = 5). The content of epicatechin was different in nine samples purchased.
CONCLUSIONThe method is good reproducible and can be used to determine epicatechin in Rhizoma fagopyri dibotoryis.
