Determination of salidroside and p-tyrosol in Hongjingtian for injection(freezing-dry) by SPE-HPLC.
- Author:
Jian MENG
1
;
Shu-Fen WANG
;
Fei HAN
;
San-Ming LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; administration & dosage; chemistry; Glucosides; analysis; Injections; Phenols; analysis; Phenylethyl Alcohol; analogs & derivatives; analysis; Plants, Medicinal; chemistry; Rhodiola; chemistry; Temperature
- From: China Journal of Chinese Materia Medica 2006;31(2):119-121
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assay salidroside and p-tyrosol in Hongjingtian for injection (freezing-dry).
METHODSamples were purified by Sep-Pak C18 column and salidroside and p-tyrosol were determined by HPLC with Irregular-H C18 (4.6 mm x 250 mm, 5 microm), and eluted with a mobile phase of methanol-acenitonitrile -0.06% phosphate (10: 10:80). The flow rate was 1.0 mL x min(-1), the detection wavelength was set at 275 nm and the column temperature was maintained at 30 degrees C.
RESULTThe calibration curves were linear in the range of 2.24-22.4 microg for salidroside (0.999 7) and 0.856-8.56 microg for p-tyrosol (0.999 6), the average recovery was 101.3%, 99.8% respectively.
CONCLUSIONThe method is convenient, rapid, accurate and reliable.
