Determination of aristolochic acid A in Guanxinsuhe preparations by RP-HPLC.
- Author:
Lin LI
1
;
Hui-Min GAO
;
Zhi-Min WANG
;
Wei-Hao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Aristolochia; chemistry; Aristolochic Acids; analysis; Capsules; Cardiotonic Agents; administration & dosage; chemistry; Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Liquidambar; chemistry; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2006;31(2):122-124
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a determination method of aristolochic acid A in Guanxisuhe preparations by RP-HPLC.
METHODThe instrument used was Hewlett-Packard 1100 HPLC with a Alltech C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase was methanol-water-acetic acid (68: 32:1) and the flow rate was 1.0 mL x min(-1). The UV detection wavelength was 390 nm and the column temperature was at 35 degrees C. The extracted solvent for the preparations was methanol solution contained 10% formic acid.
RESULTThe calibration curve was linear (r = 0.999 9) within the range of 0.119-1.89 microg for aristolochic acid A. The average recovery 99.0%, RSD 0.63%.
CONCLUSIONThe method with good linear relationship was convenient, quick, accurate, and suitable for the quality control of the aristolochic acid A in Guanxinsuhe and other traditional Chinese medicines containing aristolochic acid A.
