Effects of traditional tibetan medicine, Fructus Lonicerae microphyllae on phagecytosis and cytokines production of murine macrophages.
- Author:
Ju-Le WANG
1
;
Yang SUN
;
Hui-Ying ZHOU
;
Qiang XU
;
Zhu DUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Drugs, Chinese Herbal; isolation & purification; pharmacology; Female; Fibroblasts; cytology; Fruit; chemistry; Interferon-gamma; biosynthesis; genetics; Interleukin-1; secretion; Lonicera; chemistry; Macrophages, Peritoneal; metabolism; physiology; Mice; Mice, Inbred C57BL; Mice, Inbred ICR; Phagocytosis; drug effects; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Tumor Necrosis Factor-alpha; biosynthesis; genetics
- From: China Journal of Chinese Materia Medica 2006;31(2):145-148
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of traditional Tibetan medicine, Fructus Lonicerae microphyllae (FLM) on phagecytosis and cytokines production of murine macrophages.
METHODThe phagecytosis of murine macrophages was analyzed by neutral red phagecytosis assay. The activities of IL-1 and TNF-alpha were measured by biological methods. The mRNA of TNF-alpha and INF-gamma expressed by macrophages was detected by RT-PCR.
RESULTThe phagecytosis of murine macrophages was significantly enhanced by FLM at a concentration from 1 microg x mL(-1) to 100 microg x mL(-1) and the secretions of IL-1, and TNF-alpha from macrophages were markedly induced by FLM. Meanwhile, FLM also increased the expression of TNF-alpha mRNA and INF-gamma mRHA from macrophages in vitro.
CONCLUSIONFLM could promote phagecytosis and cytokines production of murine macrophages.
