Simultaneous determination of resveratrol, emodin, chrysophanol, physcion, in root of Polygonam cuspidatum and its extract by HPLC.
- Author:
Hong-Mei YANG
1
;
Bo CHEN
;
Jian-Guo ZENG
;
Jian-Rong MIAO
Author Information
- Publication Type:Journal Article
- MeSH: Anthraquinones; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Emodin; analogs & derivatives; analysis; Fallopia japonica; chemistry; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control; Stilbenes; analysis
- From: China Journal of Chinese Materia Medica 2006;31(3):202-205
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC method for simultaneous determination of resveratrol, emodin, chrysophanol, physcion in root of Polygonum cuspidatum and its extract.
METHODThe separation was performed in a Hypersil ODS C18 column with a mobile phase of Acetonitrile-1%HAc. The flow rate was 1.0 mL x min(-1) and detection wavelength was at 287 nm.
RESULTThe mean recovery and RSD of resveratrol, emodin, chrysophanol, physcion was 101.8%, RSD 1.7%; 96.2%, RSD 1.4%; 99.7%, RSD 1.0%; 98.60%, RSD 0.97% respectively.
CONCLUSIONThe method is accurate, simple and reliable. It can be used for quality control of P. cuspidatum and its extract.
