Determination of acetylharpagide in Ajuga decumbens by HPLC.
- Author:
Dang-Kun YUE
1
;
Guang-Zhi DING
;
Wei-Hao WANG
;
Zhi-Min WANG
;
Jian-Rong LI
;
Yan TONG
Author Information
- Publication Type:Journal Article
- MeSH: Ajuga; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Plants, Medicinal; chemistry; Pyrans; analysis; Reproducibility of Results; Sensitivity and Specificity
- From: China Journal of Chinese Materia Medica 2006;31(4):289-290
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens.
METHODThe chromatographic conditions were as follows: a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (15:85), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm.
RESULTLinearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively.
CONCLUSIONThe contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.
