Effect of ray cartilage glycosaminoglycans (RCG) on the expression of MMP-9 in Lewis lung carcinoma.
- Author:
Bin GUO
1
;
Guan-Ying HAN
;
Zhi LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; isolation & purification; pharmacology; Carcinoma, Lewis Lung; enzymology; pathology; Cartilage; chemistry; Cell Line, Tumor; Glycosaminoglycans; isolation & purification; pharmacology; Matrix Metalloproteinase 9; biosynthesis; genetics; Mice; Mice, Inbred C57BL; Neoplasm Transplantation; RNA, Messenger; biosynthesis; genetics; Random Allocation; Skates (Fish)
- From: China Journal of Chinese Materia Medica 2006;31(4):325-328
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of Ray cartilage glycosaminoglycans (RCG) on the expression of MMP-9 in Lewis lung carcinoma of mice.
METHODThe model of mice with Lewis lung carcinoma was induced. The experimental mice were randomly divided into normal saline group, RCG groups at varied concentrations and CTX group. Tumor growth state was observed, and tumor inhibitory rate of primary tumor and number of lung metastasis focus were measured. The expression of MMP-9 mRNA and protein in Lewis lung carcinoma was determined with RT-PCR and Western blot.
RESULTAs compared with normal saline group, tumor growth curves in RCG groups were smooth, there were significant differences of inhibitory rates of primary tumor and number of lung metastasis focus between RCG groups and normal saline group, and MMP-9 mRNA and protein expression levels in RCG groups were reduced significantly.
CONCLUSIONRCG can inhibit effectively the growth and metastasis of implanted Lewis lung carcinoma in C57BL/6 mice, which is probably attributed to reducing the expression of MMP-9 mRNA and protein.
