Study on sini decoction in treatment of intestinal ischemia-reperfusion injury in rats: mechanism relating to oxygen radical and bcl-2 protein.
- Author:
Ke-Xuan LIU
1
;
Wei-Kang WU
;
Wei HE
;
Hui-Lan SUN
Author Information
- Publication Type:Journal Article
- MeSH: Aconitum; chemistry; Animals; Apoptosis; drug effects; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Female; Ginger; chemistry; Glycyrrhiza uralensis; chemistry; Intestinal Mucosa; metabolism; pathology; Male; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-bcl-2; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Reperfusion Injury; metabolism; pathology
- From: China Journal of Chinese Materia Medica 2006;31(4):329-348
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of Sini decoction (SND) on intestinal mucosa in rats with intestinal ischemia reperfusion (I/R) and the mechanism relating to oxygen radical and Bcl-2 protein expression.
METHODThirty-two SD rats of both sexes were randomly divided into 4 equal groups: (1) control group in which sham operation was performed; (2) model group in which intestinal I/R was produced by clamping super mesenteric artery(SMA) for 1 hour and declamping SMA for 3 hours; (3) SND low dose group in which SND(3 g x kg(-1) x d(-1)) was given via stomach tube for 3 days before operation; (4) SND high dose group in which SND(6 g x kg(-1) x d(1)) was given via stomach tube, for 3 days before operation. A strip of small intestine was taken from distal end of ileum for light microscopic examination, Chiu's score and the detection of intestinal water content (IWC). Apoptosis of intestinal mucosa cell was examined by TUNEL method. Superoxide dismutase (SOD) activity, malondisldehyde (MDA) concentration of intestinal mucosa were detected. The protein express of Bcl-2 of intestinal mucosa was analyzed by immunochemistory.
RESULTIntestinal /R resulted in histopathological changes in intestinal mucosa, increased Chiu's scores, apoptosis index, IWC and MDA content, and reduced SOD activity and the protein expression of Bcl-2 significantly (P < 0.01). The pretreatment of SND could attenuate the above changes significantly (P < 0.01), but there was no significant difference for the above variables between SND low dose group and SND high dose group (P > 0.05). Apoptosis index was significantly negatively correlative to SOD activity in model group and two SND groups. There were significantly negative correlation between apoptosis index and protein expression of Bcl-2 in model group and SND low dose group.
CONCLUSIONSND can attenuate intestinal mucosa injury following intestinal U/R, which is related to reducing intestinal mucosa cell apoptosis by removing oxygen free radical and upregulating the protein expression of Bcl-2.
