Preparation of solid lipid nanoparticles loaded with Xionggui powder-supercritical carbon dioxide fluid extraction and their evaluation in vitro release.
- Author:
Yan-jun CHEN
1
;
Ri-xian JIN
;
Ya-qin ZHOU
;
Jing ZENG
;
He ZHANG
;
Qing-ran FENG
Author Information
- Publication Type:Journal Article
- MeSH: Angelica sinensis; chemistry; Chromatography, Supercritical Fluid; Delayed-Action Preparations; Drug Carriers; Drug Combinations; Drug Compounding; methods; Drug Delivery Systems; Drugs, Chinese Herbal; administration & dosage; isolation & purification; Ligusticum; chemistry; Nanostructures; Particle Size; Phospholipids; Poloxamer; Solubility
- From: China Journal of Chinese Materia Medica 2006;31(5):376-379
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the preparation of solid lipid nanoparticles loaded with Xionggui powder-supercritical carbon dioxide fluid extraction and their evaluation in vitro release.
METHODTo prepare solid lipid nanoparticles (SLN) loaded with Xionggui powder-supercritical carbon dioxide fluid extraction (XG-CO2-SFE) using a hot dispersion- ultrasonic technique, establishing the best prescription of XG-CO2-SFE-SLN through orthogonal design methods using entrapment efficiency of nanoparticles as index, and investigating their physicochemical characterizations. The invro release action of SLN was studied in different dissolution mediums using dynamic dialyse method.
RESULTThe best prescription was: phospholipid: F-68: stearie acid glyceride = 5: 2 : 1, the entrapment efficiency of nanoparticles was 96.3%, and the results revealed the nanoparticles were sphere like with the mean size of 245.8 nm, the mean Zeta potential was -33.5 mV. The in vitro release meet to Weibull distribution in physiological brine and to single-index model in pH 7.4 phosphate liquid (40% EtOH).
CONCLUSIONThe preparation method of the XG-CO2-SFE-SLN was appropriate, and the XG-CO2-SFE-SLN was released completely.
