Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC.
- Author:
Xu-wen LI
1
;
Ming-yu GUI
;
Ying ZHENG
;
Yong-ri JIN
;
Han-qi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Ginsenosides; analysis; chemistry; isolation & purification; Hydrolysis; Panax; chemistry; Plant Leaves; chemistry; Plant Stems; chemistry; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2006;31(5):386-388
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine 20(S)-ginsengnoside Rh2 in the hydrolysis product of saponins from leaves of Panax qinquefolium.
METHODThe separation was performed on ZORBAX EXEND C18 column (4.6 mm x 250 mm, 5 microm), eluted with methanol and water (85:15) as mobile phase with the rate of 1.2 mL x min(-1) at 25 degrees C, the wavelength for measurement was 203 nm.
RESULTThe calibration curve was linear in the range of 0.5-25 microg for 20(S)-ginsengnoside Rh2(r = 0.9999, n = 7). The average recovery was 99.7% (RSD= 1.0%).
CONCLUSIONThis method is simple, accurate, reliable and reproducible. The result shows that the transform ratio of 20(S)-ginsengnoside Rh2 is high by this hydrolysis method.
