Determination of curcumol in Rhizoma Curcumae by GC.
- Author:
Ya-er ZHU
1
;
Xiao-ping ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Gas; Curcuma; chemistry; Plants, Medicinal; chemistry; Reproducibility of Results; Rhizome; chemistry; Sesquiterpenes; analysis
- From: China Journal of Chinese Materia Medica 2006;31(5):389-390
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method to determine the content of Curcumol in Rhizoma Curcumae.
METHODThe samples were determined by GC on a HP-5 column (0.32 mm x 30 m, 0.25 micron), Inlet temperature 200 degrees C, FID 250 degrees C, flow 1.0 mL x min(-1), splitless. Temperature programming started at 60 degrees C, holding for 4 min, then increased to 210 degrees C at a rate of 3 degrees C x min(-1).
RESULTThe calibration curve of curcumol is linear over the range of 40.0-2,000 microg x mL(-1), r = 0.9997. The high and low additive recovery were 95.01% (RSD 2.52%), 96.46% (RSD 2.86%).
CONCLUSIONThis method was accurate and reliable with a good reproducibility, and the procedure of samples pretreatment is simple.
