Effects of Rehmannia glutinosa oligosaccharides on proliferation of 3T3-L1 adipocytes and insulin resistance.
- Author:
Xiao-nong GUO
1
;
Ru-xue ZHANG
;
Zheng-ping JIA
;
Mao-xing LI
;
Juan WANG
Author Information
- Publication Type:Journal Article
- MeSH: 3T3-L1 Cells; Adipocytes; cytology; Animals; Cell Proliferation; drug effects; Dexamethasone; pharmacology; Dose-Response Relationship, Drug; Insulin Resistance; Mice; Oligosaccharides; administration & dosage; isolation & purification; pharmacology; Plants, Medicinal; chemistry; Rehmannia; chemistry
- From: China Journal of Chinese Materia Medica 2006;31(5):403-407
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of Rehmannia glutinosa oligosaccharides (ROS) on the proliferation of 3T3-L1 adipocytes and insulin resistance.
METHOD3T3-L1 preadipocytes were cultured, the proliferation of 3T3-L1 preadipocytes was detected by MTT method. Insulin resistant 3T3-L1 adipocytes cell model was induced by dexamethasone and the change of glucose concentration in cell culture was determined after ROS treatment.
RESULTIn the high glucose DMEM culture media, MTT method showed that the absorbance at 570nm of 3T3-L1 preadipocytes was increased and that of 3T3-L1 adipocytes was decreased. ROS significantly increased glucose consumption in 3T3-L1 preadipocytes and adipocytes culture in a concentration-dependent manner. ROS improved the sensitivity of 3T3-L1 adipocytes to insulin.
CONCLUSIONROS can promote the proliferation of 3T3-L1 preadipocytes, inhibite the proliferation of 3T3-L1 adipocytes, and also, significantly improve insulin resistance induced by dexamethasone.
