Proteins in sperm and seminal plasma associated with human sperm resistance to cryopreservation.
- Author:
Xin-Zong ZHANG
1
;
Cheng-Liang XIONG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Cryopreservation; Humans; Male; Proteomics; Semen; metabolism; Seminal Plasma Proteins; metabolism; Sperm Motility; Spermatozoa; metabolism; Young Adult
- From:National Journal of Andrology 2013;19(3):214-217
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the proteins that could improve the resistance of human sperm to cryopreservation using comparative proteomics.
METHODSA total of 31 semen samples from 10 donors were divided into a high recovery and a low recovery group. Differentially expressed proteins in sperm and seminal plasma were detected and compared between the two groups by two-dimensional differential gel electrophoresis and mass spectrometry.
RESULTSTotally, 22 differentially expressed proteins were found in the two groups, 12 seminal plasma proteins, 9 sperm proteins, and 1 belonging to both. These identified proteins were involved in the maturation, movement, energy metabolism, DNA repair and other activities of spermatozoa.
CONCLUSIONMany proteins were identified in sperm and seminal plasma that might influence the resistance of human sperm to cryopreservation.
