Pre-freezing equilibration improves post-thaw human semen parameters.
- Author:
Ya-Nan WANG
1
;
Sha-Sha ZOU
;
Qian XIAO
;
Yong LIU
;
Xiao-Rong CAO
;
Zheng LI
;
Hong-Liang HU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Cryopreservation; methods; Humans; Male; Semen Analysis; Semen Preservation; methods; Sperm Banks; Sperm Count; Sperm Motility; Young Adult
- From: National Journal of Andrology 2013;19(10):886-889
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of pre-freezing equilibration on the cryo-survival of human sperm and to optimize the protocol of direct fumigation for the freeze-thawing of human sperm.
METHODSWe collected 50 semen samples from healthy donors, each subjected to cryopreservation with 3 different methods: non-equilibration freezing (Group A), 10-min equilibration at room temperature before freezing (Group B), and 10-min equilibration at 4 degrees C before freezing (Group C). We examined all the post-thaw semen samples by computer-assisted semen analysis for the sperm motility parameters, and detected the sperm vitality and deformity index (SDI).
RESULTSThe recovery rate of progressive sperm motility was (61.88 +/- 16.94)% in Group C, remarkably higher than in A ([48.61 +/- 16.44]%) and B ([49.41 +/- 13.77]%) (P < 0.05), but with no significant difference between the latter two. And there were no significant differences in sperm vitality and SDI among the three groups.
CONCLUSIONTen-minute equilibration at 4 degrees C before freezing can evidently improve the progressive motility of sperm in addition to its advantages of easy operation and controllable experimental condition.