Effects and mechanism of glucagon-like peptide-1 on injury of rats cardiomyocytes induced by hypoxia-reoxygenation.
- Author:
Yun XIE
1
;
Shao-xin WANG
;
Wei-wei SHA
;
Xue ZHOU
;
Wei-lin WANG
;
Li-pin HAN
;
Dai-qing LI
;
De-min YU
Author Information
- Publication Type:Journal Article
- MeSH: Actins; analysis; Animals; Butadienes; pharmacology; Cell Hypoxia; Cells, Cultured; Chromones; pharmacology; Extracellular Signal-Regulated MAP Kinases; physiology; Glucagon-Like Peptide 1; pharmacology; MAP Kinase Signaling System; Morpholines; pharmacology; Myocytes, Cardiac; drug effects; Nitriles; pharmacology; Phosphatidylinositol 3-Kinases; physiology; Rats; Rats, Wistar
- From: Chinese Medical Journal 2008;121(21):2134-2138
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDAlthough the insulinotropic role of glucagon-like peptide-1 (GLP-1) in type 2 diabetes mellitus has been substantiated, its role in cardioprotection remains largely unknown. This study aimed to determine the effects of GLP-1 on injury of rats cardiac myocytes induced by hypoxia-reoxygenation (H/R) and the possible mechanisms.
METHODSThe cultured neonatal rats cardiac myocytes were randomly divided into seven groups: the normal control group, the H/R group, the GLP-1 + H/R group, the GLP-1 + H/R + UO126 (the p42/44 mitogen-activated protein kinase (MAPK) inhibitor) group, the GLP-1 + H/R + LY294002 (phosphatidylinositol 3-kinase (PI3K) inhibitor) group, the H/R + UO126 group, and the H/R + LY294002 group. The lactate dehydrogenase (LDH) activity, apoptosis rate of cardiac myocytes, and caspase-3 activity were detected after the injury of H/R.
RESULTSCompared with the normal control group, the activity of LDH, cardiac myocyte apoptosis rate, and caspase-3 activity all increased significantly in the H/R group (P < 0.01). Compared with the H/R group, these three indices all decreased in the H/R + GLP-1 group (P < 0.01). However, the changes of LDH activity, apoptosis rate, and caspase-3 activity were inhibited by LY294002 and UO126 respectively.
CONCLUSIONSGLP-1 can directly act on cardiac myocytes and protect them from H/R injury mainly by inhibiting their apoptosis. Its mechanism may be through the PI3K-Akt pathway and the MAPK signaling pathway.
