Content comparison of buddleoside and pectolinarin in Cirsium japonicum, C. leo and C. leducei.
- Author:
Zhi-Hao LI
1
;
Liang-Ke SONG
;
Xiao-Ning WANG
;
Yan WANG
;
Shu-Xing WU
;
Cheng-Long YU
;
Yu-Ying ZONG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Chromones; analysis; chemistry; Cirsium; chemistry; Drugs, Chinese Herbal; analysis; chemistry; Reproducibility of Results; Solubility; Species Specificity
- From: China Journal of Chinese Materia Medica 2013;38(5):674-677
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare Cirsium japonicum characteristics with C. leo and C. leducei, along with the content of buddleoside and pectolinarin, and lay the foundation for the quality control of C. japonicum.
METHODSamples were collected and the relevant drugs were bought. The samples were divided into root, stem, leaf and flower, and the content of buddleoside and pectolinarin was determine by the HPLC. Chromatographic column: Waters XBridge C18 (4.6 mm x 250 mm), mobile phase: methanol-water (45: 55), measurement wavelength: 326 nm, flow rate: 0.8 mL x min(-1), column temperature: 30 degrees C. RESULT AND CONDUSION: Standard curve equation of buddleoside: Y = 74 064X-47 748, R2 = 0.991. Standard curve equation of pectolinarin: Y = 1 711 64X - 180 707, R2 = 0.999. The content of buddleoside: C. japonicum leaf was 1.987 3%, C. leo leaf 1.412 2%, C. leducei leaf 0.149 2%. The content of buddleoside was lower in root and stem. Pectolinarin was not detected in the C. japonicum and C. leo. The pectolinarin content was 0.069 0% in C. leducei leaf.
