Simultaneous determination of ephedrine hydrochloride, D-pseudoephedrine and amygdalin in xiao'er pingchuan qutan granule by HPLC.
- Author:
De-Bin YANG
1
;
Yan TONG
;
Zhen-Shan MA
;
Lin WANG
;
Mei-Hong DONG
;
Yan-Ling LI
;
Jin-Yu WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amygdalin; analysis; chemistry; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Ephedrine; analysis; chemistry; Linear Models; Pseudoephedrine; analysis; chemistry; Reproducibility of Results; Time Factors
- From: China Journal of Chinese Materia Medica 2013;38(5):687-690
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC method for the determination of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin in Xiao'er Pingchuan Qutan granule.
METHODPheny ether chromatographic column (4.6 mm x 250 mm, 5 microm) was adopted, with acetonitrile-0.1% phosphoric acid (containing 0.1% three ethylamine) (3:97) as the mobile phase. The UV detection wavelength was at 210 nm, with the flow rate of 1 mL x min(-1), and column temperature was at 35 degrees C.
RESULTThe linearity of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin ranged between 0.078 60-3.144 microg (r = 1.000 0), 0.103 4-2.068 microg (r = 0.999 7) and 0.430 5-3.157 microg (r = 0.999 8), respectively. Their average recoveries were 98.46% (RSD 1.1%), 103.0% (RSD 1.5%) and 97.15% (RSD 2.1%), respectively.
CONCLUSIONThe method is simple, stable and reliable that it can be used to determine the content of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin in Xiao'er Pingchuan Qutan granule.
