Comparison of content of total alkaloids and aconitine in Aconitum szechenyianum from different producing areas on basis of macroporous adsorption resin purification technology.
- Author:
Li LIN
1
;
Xue-Ming WEI
;
Yan-Hui WANG
;
Ling JIN
;
Zheng-Yang HE
;
Tao DU
;
Hong-Gang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Aconitine; chemistry; isolation & purification; Aconitum; chemistry; Adsorption; Alkaloids; chemistry; isolation & purification; China; Chromatography, High Pressure Liquid; instrumentation; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Porosity; Resins, Synthetic; chemistry
- From: China Journal of Chinese Materia Medica 2013;38(7):995-999
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the conditions of purifying the total alkaloids in Aconitum szechenyianum with macroporous adsorption resin, and compare the content of total alkaloids and aconitine in A. szechenyianum from different producing areas, in order to provide basis for further studies.
METHODThe orthogonal experiment method was adopted for optimizing the conditions of purifying the total alkaloids in A. szechenyianum with macroporous adsorption resin. The content of total alkaloids and aconitine were determined by using the titration method. The total alkaloids in A. szechenyianum from different producing areas were purified under optimum processing conditions. Aconitine was determined by HPLC.
RESULTDifferent processing conditions showed different influences on the purification of total alkaloids. The optimum conditions were resin type HPD-722, ethanol concentration of 80% , and ethanol elution volume of 80 mL x min(-1). The contents of aconitine in A. szechenyianum from different producing areas--Qinghai, Maxianshan, Ningxia and Yongdeng were 0.493 5, 0.883 5, 1.527 8, 1.664 4 mg x g(-1), respectively.
CONCLUSIONThe optimum processing conditions used in this essay could be used for purifying the total alkaloids and aconitine. A. szechenyianum from Yongdeng and Gansu contains the highest content of aconitine.
