OBJECTIVETo establish an HPLC fingerprint of ethyl acetate extraction of Saxifraga stolonifera.

METHODThe HPLC analysis was performed on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column with isocratic elution of acetonitrile-0.05% phosphoric acid at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 256 nm and the column temperature was set at 30 degrees C.

RESULTThe HPLC fingerprint of ethyl acetate extraction of S. stolonifera has been established. There were fifteen common peaks, seven of which were identified by reference substances. The RSD of relative retention time was less than 3% in the precision and repeated tests. Eleven samples from different area can be distinguished from their fingerprints.

CONCLUSIONThis method is reasonable and reliable and can be used for quality control of S. stolonifera.