Study on tissue distribution of borneol in mice by intravenous and intranasal administrations.
- Author:
Jing-Yi ZHAO
1
;
Shou-Ying DU
;
Yang LU
;
Hui-Chao WU
;
Hui-Yun LI
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Intranasal; methods; Administration, Intravenous; methods; Animals; Bornanes; administration & dosage; pharmacokinetics; Drugs, Chinese Herbal; administration & dosage; pharmacokinetics; Female; Mice; Mice, Inbred ICR; Tissue Distribution
- From: China Journal of Chinese Materia Medica 2013;38(7):1071-1074
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a GC-FID method to determine borneol's concentration in mouse tissues, and to investigate the tissue distribution after intravenous and intranasal administrations of borneol.
METHODMouse brains, hearts, livers, spleens, lungs and kidneys were collected at 1, 3, 5, 10, 20, 30, 60, 90, 120 min after administration of borneol with the dose of 30.0 mg x kg(-1). The drug in tissues was extracted with ethyl acetate, and borneol's concentration detected by GC, with octadecane as the internal standard.
RESULTThe calibration curve showed a good linear relationship. Extraction recoveries, inter-day and intra-day precisions and stability were in conformity with the analytical requirements of biological samples. Borneol was mainly distributed in most tissues, more in heart, brain and kidney, and less in liver, spleen and lung.
CONCLUSIONThe established GC-FID method is applicable for content determination of borneol in tissues. After intravenous and intranasal administrations in mice, borneol is mainly distributed in abundant blood-supply tissues. After intranasal administration, brain tissues showed the highest target coefficient and target effectiveness.
