Study on intestinal absorption of tilianin in rats of single-pass perfusion model.
- Author:
Ting-Ting WANG
1
;
Wei LI
;
Yong YUAN
;
Li-Ping WANG
;
Xin-Chun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Caco-2 Cells; Drugs, Chinese Herbal; pharmacokinetics; Female; Flavonoids; pharmacokinetics; Glycosides; pharmacokinetics; Humans; Intestinal Absorption; Lamiaceae; chemistry; Male; Models, Biological; Perfusion; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2013;38(7):1079-1082
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the intestinal absorption mechanism of tilianin in rats.
METHODThe single-pass perfusion model was established in rats. The concentrations of tilianin with in situ intestinal perfusion were determined by HPLC. The impact factors, such as verapamil, reserpine, phloridzin and rifampicin, on Ka and Papp of tilianin in rat jejunum were investigated.
RESULTCompared with the control group, Ka and Papp in rat jejunum were significantly higher after being added with verapamil and reserpine (P < 0.05). Papp of tilianin in rat jejunum was significantly lower after being added with phloridzin (P < 0.05). Compared with the control group, both Ka and Papp of tilianin in rat jejunum were not significantly higher after being added with rifampicin.
CONCLUSIONTilianin is the substrate of P-gp, BCRP and SGLT1. The effluent effect of P-gp and BCRP is the main mechanism of tilianin in intestinal absorption, indicating that tilianin can realize intestinal absorption and transport by relying on SGLT1. Tilianin is not the substrate of bile salt transporter protein.
