Accurate identification of Psammosilene tunicoides and its confused species by systematic identification method.
- Author:
Cai-Li LIAO
1
;
Chun-Sheng LIU
;
Yuan-Yuan ZHANG
;
Hao-Zhong WU
;
Xue-Yong WANG
;
Xiao-Li CHENG
;
Guo-Jie XU
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Caryophyllaceae; chemistry; classification; cytology; genetics; DNA, Intergenic; Phenotype; Phylogeny; Sequence Alignment
- From: China Journal of Chinese Materia Medica 2013;38(8):1134-1137
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an effective identification method for accurately discriminating Psammosilene tunicoides and its confused species by the combined method of microscopic identification and molecular identification, so-called systematic identification of Chinese materia medica (SICMM).
METHODP. tunicoides and its confused species were accurately discriminated by SICMM method, which was established by comprehensively use of microscopic identification and DNA identification method. The DNA identification included the following analysis: the BLAST alignment, specific bases and N-J phylogenetic tree analysis.
RESULTThe cluster crystals were not observed in P. tunicoides, but great deals of them were found in Silene viscidula. Further more, big differences of ITS sequence were observed and analyzed between P. tunicoides and its confused specie of S. viscidula.
CONCLUSIONThe system method is a scientific and accurate method for the identification of P. tunicoides and its counterfeit species.