The expressions of P38 mitogen-activated protein kinases and JNK mitogen-activated protein kinases in the renal tissue of rats with acute paraquat poisoning and the therapeutical effects of melatonin.
- Author:
Xin WANG
1
;
Yanxia ZHAO
;
Haixia LI
;
Shuang LIU
;
Yingping TIAN
2
;
Aijun LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; JNK Mitogen-Activated Protein Kinases; metabolism; Kidney; drug effects; metabolism; physiopathology; Male; Melatonin; therapeutic use; Paraquat; poisoning; Rats; Rats, Sprague-Dawley; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(5):366-369
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expressions of P38 mitogen-activated protein kinases (P38 MAPK), JNK mitogen-activated protein kinases (JNK MAPK) and the therapeutical effects of melatonin in the renal tissue of acute acuteparaquat-induced rats.
METHODSSeventy-eight healthy adult Sprague-Dawley (SD) rats (39 male, 39 female) were randomly divided into three groups: (1) Control group (group A): 6 rats, (2) Poisoned group (group B): 36 rats, (3) Therapeutical group (group C): 36 rats. At 1, 3, 5, 7, 10 and 14 days after poisoning, six rats in Group B and group C were used to assess renal pathological changes and the expression of P38 MAPK, JNK MAPK in kidney were evaluated by immunohistochemistry.
RESULTSCompared with control group, the expression of P38MAPK in renal tissue of poisoned group significantly rose at the first day, reached the peak at the 10th day and afterwards decreased slowly. Expression of JNK MAPK reached the peak at the first day, and kept at relatively high levels up to the 14th day. Melatonin weakened markedly the expressions of P38 MAPK and JNK MAPK in renal tissue of acute acuteparaquat-induced rats.
CONCLUSIONP38 MAPK and JNK MAPK play an important role in renal injury of acute paraquat -poisoning rats. Melatonin takes a significant effect on the activation of them.
