Gene variance in microsomal epoxide hydrolase and the susceptibility of coal workers' pneumoconiosis.

Congcong Chen; Lijuan Wang; Jingjin Yang; Ting Wang; Xiaoming JI; Baiqun WU; Ruhui Han; Chunhui NI; E-mail: Chni@njmueducn

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Gene variance in microsomal epoxide hydrolase and the susceptibility of coal workers' pneumoconiosis.

Author: Congcong CHEN ¹ ; Lijuan WANG ¹ ; Jingjin YANG ¹ ; Ting WANG ¹ ; Xiaoming JI ¹ ; Baiqun WU ¹ ; Ruhui HAN ¹ ; Chunhui NI ² ; E-mail: CHNI@NJMU.EDU.CN.
Author Information

1. Department of Occupational Medicine and Environmental Health, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
2. Department of Occupational Medicine and Environmental Health, School of Public Health, Nanjing Medical University, Nanjing 210029, China
Publication Type:Journal Article
MeSH: Anthracosis; genetics; Case-Control Studies; Coal; Epoxide Hydrolases; genetics; Genetic Predisposition to Disease; Genotype; Humans; Polymorphism, Single Nucleotide; Risk Factors; Sequence Analysis, DNA
From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(7):492-495
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore whether the tagging single nucleotide polymorphisms (SNPs) within EPHX1 gene were involved in the genetic susceptibility to coal worker's pneumoconiosis (CWP) by case-control study.
METHODSThis study consisted of 697 CWP patients and 694 controls. All the subjects were Han Chinese, underground coal miners and recruited from coal mines of Xuzhou Mining Business Group Co Ltd.. The venous blood samples were obtained from all subjects and extracted genome DNA from the isolated leucocytes. Three SNPs were selected from the HapMap and the genotyping was done by the TaqMan method with the ABI 7900HT Real Time PCR system.
RESULTSThe Single SNP analyses showed that the genotype frequencies of EPHX1 (rs2234922) was significantly associated with decreased risk of CWP under co-dominant model (OR = 0.22, 95% CI = 0.06~0.79, P = 0.020), recessive model (OR = 0.23, 95% CI = 0.06~0.82, P = 0.023), and addictive model (OR = 0.75, 95% CI = 0.58~0.96, P = 0.022). The further stratification analysis showed that the risk of CWP will significantly decreased in non-smoking groups (OR = 0.10, 95% CI = 0.01~0.83, P = 0.033).
CONCLUSIONSOur results suggest that individuals with the EPHX1 (rs223492) GG genotype was associated with a dereased risk of CWP, and it has a protective effect on the developing CWP.