Effect of chronic aluminum exposure on neuron apoptosis and expression of P53 phosphorylation in rats.
- Author:
Baolong PAN
1
;
Shuandong GUO
;
Liang GUO
;
Xin LI
;
Qiao NIU
2
Author Information
- Publication Type:Journal Article
- MeSH: Aluminum; toxicity; Aluminum Compounds; toxicity; Animals; Apoptosis; Cerebral Cortex; metabolism; Chlorides; toxicity; Male; Neurons; cytology; drug effects; Phosphorylation; Rats; Rats, Sprague-Dawley; Tumor Suppressor Protein p53; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(7):532-534
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of P53 phosphorylation in neuron apoptosis of rats by chronic aluminum exposure.
METHODSA total of male 40 SD rats were divided randomly into 4 groups (n = 10/dose), the exposed groups were fed with normal diet with different concentration of AlCl3 · 6H2O for 6 months respectively. The dosage of low, middle and high groups were 10.73, 107.33, 1073.33 mg/kg in sequence. The control group received normal diet. The neuron apoptosis was measured by method of Tunel. The expressions of P53 and pP53-ser15 protein in the cortex were detected by Western-blot.
RESULTSTunel staining showed that the low, middle and high group rats had increased apoptosis rate than control group (P < 0.01). Western-blot test demonstrated that the expression of P53 protein in the cortex of high group rats were significantly higher than the control and low groups (P < 0.05). The expression of pP53-ser15 protein in the cortex of middle and high group rats were also higher than the control and low groups (P < 0.05).
CONCLUSIONChronic aluminum exposure can lead to over expression of P53 and pP53-ser15 protein in cerebral cortex, which maybe one of the most important mechanisms of neuron apoptosis induced by AlCl3.
