Effects of indium on micronucleus formation in human peripheral blood lymphocytes.
- Author:
Yan GUO
1
;
Changye HUI
;
Liuzhuo ZHANG
;
Lili WANG
;
Dianpeng WANG
;
Xueqin YANG
;
Xinyue YANG
;
Zhimin LI
2
Author Information
- Publication Type:Journal Article
- MeSH: Cell Nucleus; metabolism; Cytokinesis; DNA Damage; Humans; In Vitro Techniques; Indium; toxicity; Lymphocytes; drug effects; Oxidative Stress
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(8):563-565
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the cytotoxicity of indium chloride (InCl₃) and its effects on micro-nucleus formation in primary human lymphocytes cultured in vitro.
METHODSThe CCK-8 assay was used to evaluate the cytotoxicity of 24 h exposure to different concentrations of InCl₃(4, 40, 80, 200, 500, and 1 000 µmol/L) in lymphocytes cultured in vitro. The cytokinesis-block method was used to determine the micronucleus level in lymphocytes exposed to different concentrations of InCl₃and the effects of anti-oxidant vitamin C on micronucleus frequency.
RESULTSLymphocytes exposed to InCl₃of no less than 500 µmol/L had significantly lower survival rates than those in the control group (P < 0.05). Lymphocytes exposed to 80 µmol/L InCl₃had a significantly higher micronucleus frequency than those in the control group (P < 0.05). However, there was no further increase in micronucleus frequency of lymphocytes exposed to 200 µmol/L InCl₃. Lymphocytes cultured in whole blood and exposed to 500 or 1000 µmol/L InCl₃had a significantly increased micronucleus frequency than those in the control group (P < 0.001). The increase in micronucleus frequency of lymphocytes induced by indium could be partially antagonized by 20 or 100 µmol/L vitamin C.
CONCLUSIONInCl₃can induce an increase in micronucleus frequency of primary human lymphocytes cultured in vitro, which might be associated with DNA damage induced by oxidative stress.