Inhibitory Effects of Iranian Thymus vulgaris Extracts on in Vitro Growth of Entamoeba histolytica.
10.3347/kjp.2008.46.3.153
- Author:
Maryam BEHNIA
1
;
Ali HAGHIGHI
;
Hossein KOMEYLIZADEH
;
Seyyed Javadi Seyyed TABAEI
;
Alireza ABADI
Author Information
1. Laboratory Complex, Science and Research Branch, Islamic Azad University, Tehran, Iran. alihaghi2000@yahoo.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Entamoeba histolytica;
Thymus vulgaris;
antiamebic;
Iran
- MeSH:
Animals;
Antiprotozoal Agents/chemistry/*pharmacology;
Dose-Response Relationship, Drug;
Entamoeba histolytica/*drug effects;
Iran;
Metronidazole/pharmacology;
Microbial Sensitivity Tests;
Plant Extracts/chemistry/*pharmacology;
Plant Oils/chemistry/pharmacology;
Thymus Plant/*chemistry
- From:The Korean Journal of Parasitology
2008;46(3):153-156
- CountryRepublic of Korea
- Language:English
-
Abstract:
One of the most common drugs used against a wide variety of anaerobic protozoan parasites is metronidazole. However, this drug is mutagenic for bacteria and is a potent carcinogen for rodents. Thymus vulgaris is used for cough suppression and relief of dyspepsia. Also it has antibacterial and antifungal properties. The aim of this study was to investigate antiamebic effect of Thymus vulgaris against Entamoeba histolytica in comparison with metronidazole. One hundred gram air-dried T. vulgaris plant was obtained and macerated at 25 degrees C for 14 days using n-hexane and a mixture of ethanol and water. For essential oil isolation T. vulgaris was subjected to hydrodistillation using a clevenger-type apparatus for 3 hr. E. histolytica, HM-1: IMSS strain was used in all experiments. It was found that the minimal inhibitory concentration (MIC) for T. vulgaris hydroalcoholic, hexanic extracts, and the essential oil after 24 hr was 4 mg/mL, 4 mg/mL, and 0.7 mg/mL, respectively. After 48 hr the MIC for T. vulgaris hydroalcoholic and hexanic extracts was 3 and 3 mg/mL, respectively. Therefore, it can be concluded that the Iranian T. vulgaris is effective against the trophozoites of E. histolytica.
