Effect of astragalus membranaceus on the proliferation, osteogenic capacity and structure of periodontal ligament cells in vitro.
- Author:
Chao-liang ZHANG
1
;
Xiang-li KONG
;
Si-xiu CHEN
;
Xiao-yu LI
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; Astragalus membranaceus; Cell Differentiation; Cell Proliferation; In Vitro Techniques; Periodontal Ligament
- From: West China Journal of Stomatology 2010;28(5):556-559
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Astragalus membranaceus (APS) on the proliferation, osteogenic capacity and structure of periodontal ligament cells (PDLCs) in vitro.
METHODSPDLCs were cultured in vitro with APS of 0.08, 0.1, 0.2, 0.4 mg x mL(-1). Methyl thiazolyl tetrazolium (MTr), alkaline phosphatase (ALP) and cell structure were detected to determine the proliferation and differentiation of PDLCs proliferation and differentiation.
RESULTSWhen the APS was 0.2 mg x mL(-1), the absorbance of MTT and ALP exhibit significantly increased as compared to the control (P < 0.05). The cells cultured in vitro with APS of 0.2 mg x mL(-1) had the normal structure.
CONCLUSIONAPS with proper concentration in short-term culture may promote the proliferation and differentiation of PDLCs.
