The effects of oxymatrine on expression of interleukin-6 and interleukin-1beta mRNA of human periodontal ligament cell stimulated by lipopolysaccharides.
- Author:
Yun WU
1
;
Ling CHEN
;
Kai LUO
;
Fu-hua YAN
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; Humans; Interleukin-1beta; Interleukin-6; Lipopolysaccharides; Periodontal Ligament; Quinolizines; RNA, Messenger
- From: West China Journal of Stomatology 2010;28(6):656-659
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of oxymatrine on the expression of interleukin-6 (IL-6), interleukin-1beta (IL-1beta) mRNA of human periodontal ligament cell (PDLC) stimulated by lipopolysaccharides (LPS), and to discuss oxymatrine's inhibition mechanism on periodontal inflammation stimulated by LPS.
METHODSFirstly, isolate PDLC externally and culture them; for oxymatrine experimental group we used different combination of LPS and oxymatrine in different concentration, and for the matched group we use DEME nutrient solutions of 1% FBS. Then reverse transcription-polymerase chain reaction (RT-PCR) for checking the level of IL-6 and IL-1beta mRNA.
RESULTS25 microg x mL(-1) LPS can significantly enhance the expression of both IL-6 and IL-1beta mRNA's level, and oxymatrine could restrain above phenomena.
CONCLUSIONOxymatrine can restrain the expression of IL-6 and IL-1beta mRNA of human PDLC stimulated by LPS.
