Simultaneous determination of ten major compounds including iridoid glycosides and phenolic acids in Pterocephalus hookeri by UPLC-PDA.
10.19540/j.cnki.cjcmm.20170224.015
- Author:
Ce TANG
1
;
Jian WEN
1
;
Jing WANG
1
;
Ke-Hui ZHAO
1
;
Gang FAN
1
;
Xian-Li MENG
1
;
Zhong-Mei ZOU
2
;
Yi ZHANG
1
Author Information
1. College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
2. Institute of Mechcinal Plant Development, Chinese Acadeuy of Medical Scieuce and Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
Pterocephalus hookeri;
Tibetan medicine;
UPLC-PDA;
content determination;
iridoid glycoside;
phenolic acid
- From:
China Journal of Chinese Materia Medica
2017;42(7):1234-1237
- CountryChina
- Language:Chinese
-
Abstract:
This study is to develop an UPLC-PDA method for determination of 10 major components in Pterocephalus. The UPLC-PDA assay was performed on a Waters Acquity UPLCR BEH C₁₈（2.1 mm ×100 mm,1.7 μm）, and the column temperature was at 30 ℃. The mobile phase consists of water containing 0.2% phosphoric acid (A) and acetonitrile (B) in gradient elution at a flow rate of 0.4 mL•min⁻¹. The detection wave length was set at 237 and 325 nm, and the injection volume was 1 μL in the UPLC system. The linear range of 10 detected compounds were good (r≥0.999 7), and the overall recoveries ranged from 96.30% to 103.0%, with the RSD ranging from 0.72% to 2.9%. The method was simple, accurate and reproducible, which can be used for the simultaneous determination of the content of ten major components in P. hookeri.
