Cloning and protein expression analysis of monoterpene synthase gene TwMS in Tripterygium wilfordii.
10.19540/j.cnki.cjcmm.2017.0058
- Author:
Tian-Yuan HU
1
;
Ping SU
2
;
Yi-Feng ZHANG
1
;
Hong-Yu GUAN
1
;
Jia-Wei ZHOU
1
;
Yu-Ru TONG
1
;
Wei GAO
1
;
Lu-Qi HUANG
2
Author Information
1. Key Laboratory of Collateral Diseases, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
2. State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Tripterygium wilfordii;
bioinformatics analysis;
mRNA expression analysis;
monoterpene synthases;
protein expression
- From:
China Journal of Chinese Materia Medica
2017;42(7):1312-1318
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we cloned a monoterpene synthases, TwMS from Tripterygium wilfordii suspension cells. TwMS gene contained a 1 797 bp open reading frame (ORF), encoding a polypeptide of 579 amino acids, which deduced isoelectric point (pI) was 6.10 and the calculated molecular weight was 69.75 kDa. Bioinformation analysis showed that the sequence of TwMS was consistent with the feature of monoterpene synthases. Differential expression analysis revealed that the relative expression level of TwMS increased significantly after being induced by methyl jasmonate (MeJA). The highest expression level occurred at 24 h. TwMS protein was successfully expressed in Escherichia coli BL21 (DE3), which laid the foundation for identifying the function of T. wilfordii monoterpene synthases.
