Validation of in situ single pass perfusion model based on P-gp.
10.19540/j.cnki.cjcmm.20170223.007
- Author:
Yue-Liu DONG
1
;
Yang LIU
1
;
Xiu-Wen YIN
1
;
Meng PAN
1
;
Xue-Lian LI
1
;
Zi-Yu WANG
1
;
Ling DONG
1
Author Information
1. Beijing University of Chinese Medicine, Beijing 100102, China.
- Publication Type:Journal Article
- Keywords:
P-gp;
digoxin;
in situ rat intestinal perfusion;
phenol red
- From:
China Journal of Chinese Materia Medica
2017;42(8):1539-1544
- CountryChina
- Language:Chinese
-
Abstract:
To validate in situ rats intestinal single pass perfusion model based on P-glycoprotein (P-gp). Firstly, phenol red perfusion was carried out to verify the close connection structure of intestinal epithelial cells, and the integrity of the intestinal epithelium, with a gravimetric method for correcting water flux. The level of phenol red was determined by high performance liquid chromatography (HPLC) both before and after perfusion. Secondly, the positive drug digoxin specified by FDA was used to validate the model. After different mass concentrations of verapamil were given in the rats, the absorption parameters of digoxin in ileum of rats were observed and compared. The results showed that the phenol red was absorbed in rats ileum segment, with an effective permeability coefficient of (1.09±0.62)×10 ⁻⁶ cm•s ⁻¹. The experiment results indicated that the close connection structure of intestinal epithelial cells was normal, and the integrity of the intestinal epithelium was maintained well. In digoxin perfusion experiment, in case no verapamil was given, digoxin showed certain degree of absorption in rat ileum, with an effective permeability coefficient (Peff) of (1.07±0.59)×10 ⁻⁵ cm•s ⁻¹; after mass concentrations of 0.01,0.1 mmol•L ⁻¹ verapamil were given, the absorption of digoxin was on the rise in rat ileum, with an effective permeability coefficient Peff of (1.58±0.69)×10 ⁻⁵, (3.28±0.95)×10 ⁻⁵ cm•s ⁻¹ respectively (P<0.05). Digoxin perfusion experiment verified that P-gp expression in small intestine epithelium was intact and can be used in the research of P-gp efflux transporter.
