Antiplatelet aggregation bioactivity of Polygoni Multiflori Radix with chemical fingerprints and spectrum-effect correlation analysis.
10.19540/j.cnki.cjcmm.20170301.001
- Author:
Qin HE
1
;
Can TU
2
;
Jia-Bo WANG
2
;
Zhen-Jie LIU
2
;
Meng-Chen SHA
2
;
Le ZHANG
2
;
Chun-Yu LI
2
;
Xiao-He XIAO
2
Author Information
1. School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
2. China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing 100039, China.
- Publication Type:Journal Article
- Keywords:
Polygoni Multiflori Radix;
anti-platelet aggregation;
chemical fingerprints;
spectrum-effect correlation;
stilbene glucoside
- From:
China Journal of Chinese Materia Medica
2017;42(9):1679-1684
- CountryChina
- Language:Chinese
-
Abstract:
To explore the active substance of antiplatelet aggregation of Polygoni Multiflori Radix by using chemical fingerprints and antiplatelet aggregation bioactivity test for spectrum-effect correlation analysis. The Polygoni Multiflori Radix was tested by antiplatelet aggregation in vitro, and the results showed that 50% aqueous ethanol extract of Polygoni Multiflori Radix had more potent antiplatelet aggregation effect than 10% or 90% aqueous ethanol extract, and ultrasonic extraction was superior to refluxing extraction in the aspect of antiplatelet aggregation. The antiplatelet aggregation bioactivity of the different Polygoni Multiflori Radix extracts was evaluated and the results showed that the inhibition rate was 32.03%-74.56%. Spectrum-effect correlation analysis indicated that trans-stilbene glucoside, cis-stilbene glucoside and catechinic acid had higher correlation coefficient and they were 0.963 (P<0.01), 0.902 (P<0.01) and 0.656 (P<0.05) respectively; furthermore, all of the above three compounds demonstrated significant antiplatelet aggregation bioactivities. Considering their content difference in Polygoni Multiflori Radix, we calculated the relative active contributions, and the results suggested that trans-stilbene glucoside was the main active substance of Polygoni Multiflori Radix in the aspect of antiplatelet aggregation in vitro.
