Model of B immunoblastic lymphomas in the Hu-PBL-SCID mice.
- Author:
Dong-qing CHEN
1
;
Lian-jun BAI
;
Qing-feng LIU
;
Lian-xian CUI
;
Wei HE
;
De-nian BA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Herpesvirus 4, Human; physiology; Humans; Lymphoma, Large-Cell, Immunoblastic; Mice; Mice, SCID
- From: Acta Academiae Medicinae Sinicae 2003;25(3):294-296
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo constitute a model of B immunoblastic lymphomas in the Hu-PBL-SCID mice.
METHODSThe SCID mice were reconstituted by intraperitoneal injection (i.p.) of 5 x 10(7) human lymphocytes from Epstein-Barr virus (EBV) seronegative individuals. After one week, the SCID mice were inoculated with EBV by i.p. injection, and subjected to the investigation of whether there was any tumor in the abdomen of such SCID mice four weeks later. The characteristics of the found tumor was observed by the methods of Hematoxylin-eosin (HE) stain, immunohistochemical staining and polymerase chain reaction (PCR).
RESULTSCompared with the control groups, all the EBV-infected Hu-PBL-SCID mice had abdominal solid tumors [(32 +/- 12.5) mm3] developed, often located in the liver. HE staining and immunohistochemical staining showed the tumors were human B cell lymphomas. EBV DNA could be detected in the tumors by the PCR.
CONCLUSIONSThe model of B immunoblastic lymphomas in the Hu-PBL-SCID mice is successfully constituted, and may well be useful to the human tumor immunological study.
