Effect of AP-1 on expression of plasminogen activator inhibitor-1 in human vascular endothelial cells.
- Author:
Xiao-dong LI
1
;
Shu-yu ZU
;
Wen WANG
;
Guang-jin ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Cells, Cultured; Endothelium, Vascular; cytology; metabolism; Humans; Molecular Sequence Data; Plasminogen Activator Inhibitor 1; biosynthesis; genetics; Promoter Regions, Genetic; Transcription Factor AP-1; genetics; metabolism; Tumor Necrosis Factor-alpha; genetics; metabolism; Umbilical Veins; cytology
- From: Acta Academiae Medicinae Sinicae 2003;25(3):307-311
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of nuclear transcription factor AP-1 on tumor necrosis factor alpha (TNF-alpha) or minimal modified low density lipoprotein (mmLDL)-induced expression of plasminogen activator inhibitor-1 (PAI-1) in human vascular endothelial cells.
METHODSUsing gene recombination techniques, four luciferase reporter gene plasmids containing different length of human PAI-1 gene promoter were constructed. Through the transient transfection analysis, the roles of AP-1 element (from -823 bp to -553 bp) in PAI-1 promoter have been determined. In order to further verify the role of AP-1 element, the three site-directed mutants were recovered using PCR and sequencing assay.
RESULTSThe induction by TNF-alpha or mmLDL were decreased markedly when the three AP-1 elements in PAI-1 promoter had been mutated respectively.
CONCLUSIONSThese results indicate that the AP-1 element in PAI-1 promoter may have important role in PAI-1 gene transcriptions in endothelial cells induced by TNF-alpha or mmLDL.
