Detection of SARS-CoV RNA in stool samples of SARS patients by nest RT-PCR and its clinical value.
- Author:
Yi REN
1
;
Hui-guo DING
;
Qing-fa WU
;
Wei-jun CHEN
;
Dong CHEN
;
Zhi-ying BAO
;
Ling YANG
;
Chun-hui ZHAO
;
Jian WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Feces; virology; Female; Humans; Male; Middle Aged; RNA, Viral; analysis; Reverse Transcriptase Polymerase Chain Reaction; methods; SARS Virus; isolation & purification; Severe Acute Respiratory Syndrome; transmission; virology; Time Factors
- From: Acta Academiae Medicinae Sinicae 2003;25(3):368-371
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate of severe acute respiratory syndrome (SARS) convalescent stool shedding by RT-PCR.
METHODSOne hundred and three stool samples from 46 SARS patients were collected on May 16th, 20th, and 23rd, 2003. For each sample, RNA was extracted using commercial kit and 7 Nest RT-PCR using a 14-pair different SARS-associated coronavirus (SARS-CoV) special primers were carried out simultaneously.
RESULTSAmong these 46 SARS patients, 17 cases (37.0%) were stool SARS-CoV RT-PCR negative, and 29 cases (63.0%) were SARS-CoV RT-PCR positive. The duration of positive cases lasted (31.76 +/- 10.78) d (12-64 d). The longest stool shedding case in this study lasted 64 days. Two serial stool samples and for each sample 2 RT-PCR tests using different primers were positive in this case.
CONCLUSIONSOur study observed longest stool shedding of SARS patients to be 64 days after initial onset of SARS. The average stool shedding was 32 days. Hence it is important to think highly of SARS convalescent patient stool sterilization.
