Expression of Fas, FasL, and NF-kappa B in the process of osteoclast-like cell apoptosis effected by sodium fluoride.
- Author:
Yuan-ming SUN
1
;
Fu-jun YANG
;
Yu-min LI
;
Biao LU
;
Mei ZHU
;
Ming-cai QIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Fas Ligand Protein; Female; Fluorides; pharmacology; Ligands; Membrane Glycoproteins; metabolism; Mice; Mice, Inbred C57BL; NF-kappa B; metabolism; Osteoclasts; cytology; metabolism; fas Receptor; metabolism
- From: Acta Academiae Medicinae Sinicae 2002;24(5):491-494
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the changes in the expression of apoptosis signals: Fas, FasL and NF-kappa B in the process of osteoclast-like cell (OLC) apoptosis effected by sodium fluoride.
METHODSAfter co-culture of osteoclast-like cells with 0, 5, 10 and 15 mg/L sodium fluoride, Fas, FasL and NF-kappa B antibody expressions were detected by immune-histochemistry.
RESULTSThe expression of Fas and FasL increased with the concentration of the sodium fluoride, however the expression of NF-kappa B decreased with the concentration of sodium fluoride.
CONCLUSIONIn the process of OLC apoptosis induced by sodium fluoride, the expression of Fas and FasL increased, and that of NF-kappa B decreased with the concentration of sodium fluoride respectively, and the changes of the expression present a dose-dependent pattern.
