Preliminary studies on the mechanisms of a new anti-tumor agent PH II-7 with special preference to multidrug resistant tumor cells.
- Author:
Yao-hong TAN
1
;
Jing QI
;
Wei-jun LIU
;
Chun-zheng YANG
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; biosynthesis; genetics; Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Cell Division; Drug Combinations; Drugs, Chinese Herbal; chemistry; pharmacology; HL-60 Cells; Humans; K562 Cells
- From: Acta Academiae Medicinae Sinicae 2002;24(2):134-139
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the anti-tumor activity of PH II-7 in vitro and explore preliminarily its mechanisms.
METHODSThe anti-tumor activity was measured using colorimetric MTT assay. Apoptosis was determined with fluorescence-activated cell sorter (FACS), electron microscopy and agarose gel electrophoresis. The expressions of mdr1 and sorcin genes were determined by Northern blot assay.
RESULTSPH II-7 inhibited the proliferation of various human tumor cells derived from different tumor cell lines. The IC50 values varied from 0.34-18.61 mumol/L. Especially, PH II-7 had strong inhibitory effect on multidrug resistant tumor cells, whereas adriamycin (ADR) was resistant. Apoptosis was induced in HL60 and HL60/ADR cells treated with 1 microgram/ml PH II-7, while PH II-7 inhibited the expressions of mdr1 and sorcin genes.
CONCLUSIONSPH II-7 is a new potential agent which has strong inhibitory effect on both multidrug resistant cells and their parental cells. PH II-7 may increase the intracellular drug concentration in MDR cells by inhibiting the expressions of the MDR-related genes mdr1 and sorcin and induce the apoptosis of MDR cells and their parental tumor cells.
