The transcriptional regulation study on human delta globin gene with CAAT box C-->T point mutation in its promoter.
- Author:
Jie YAO
1
;
Song-sen CHEN
;
Ke-gong YANG
;
Xu DI
;
An-qi XIONG
;
You-hong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: CCAAT-Enhancer-Binding Proteins; genetics; Globins; genetics; Humans; Point Mutation; Promoter Regions, Genetic; genetics; Transcription, Genetic
- From: Acta Academiae Medicinae Sinicae 2002;24(2):140-143
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the transcriptional regulation of human delta globin gene with C-->T point mutation at -64 in its promoter.
METHODSHuman delta globin genes including wild CAAT box and mutant CAAT box (-64C-->T) were separately cloned into eukaryotic expression vector pcDNA3.1 (-)/Myc-His A which was cut out the strong promoter CMV, transfected MEL cells, and induced by DMSO to express. The transcriptional regulation of human delta globin gene was analysed using semi-quantitative RT-PCR.
RESULTSThe expression level of human delta globin gene with mutant CAAT box was 2.2-fold as high as that with wild CAAT box.
CONCLUSIONThe defective CAAT box of human delta globin gene promoter region may be one of the major reasons for its low expression level.
