Effects of TNF-alpha or mmLDL on PAI-1 expression in human umbilical vein endothelial cells.
- Author:
Xiao-dong LI
1
;
Shu-yu ZU
;
Guang-jin ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Endothelium, Vascular; cytology; metabolism; Female; Humans; Lipoproteins, LDL; pharmacology; Mitogen-Activated Protein Kinases; metabolism; Plasminogen Activator Inhibitor 1; genetics; metabolism; RNA, Messenger; metabolism; Tumor Necrosis Factor-alpha; pharmacology; Umbilical Veins; cytology
- From: Acta Academiae Medicinae Sinicae 2002;24(2):156-159
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo demonstrate the changes of PAI-1 activity and mRNA expressions induced by tumor necrosis factor alpha (TNF-alpha) or minimally modified low-density lipoprotein (mmLDL) in human umbilical vein endothelial cells (HUVECs).
METHODSThe PAI-1 activity in HUVECs culture medium was measured by chromogenic assay. The PAI-1 mRNA expressions were determined by Northern blot. The mitogen-activated protein kinase-kinase (MAPKK) inhibitor and protein kinase C (PKC) inhibitor were used to intervene the inductions by TNF-alpha or mmLDL.
RESULTSThe PAI-1 activity and mRNA level were increased when HUVECs were exposed to TNF-alpha or mmLDL. The MAPKK inhibitor (PD98059, 60 mumol/L) could markedly inhibit PAI-1 activity and mRNA expression induced by TNF-alpha (100 U/ml) or mmLDL (50 micrograms/ml). But Staurosporine (10 nmol/L) and H7 (15 mumol/L), which were the PKC inhibitors, had no significant inhibition effect.
CONCLUSIONS(1) TNF-alpha or mmLDL could induce PAI-1 activity and mRNA expression in HUVECs. (2) Increase of PAI-1 activity induced by TNF-alpha and mmLDL was related to its mRNA expression. (3) The MAPK pathway may play a role in PAI-1 expression induced by TNF-alpha or mmLDL.
